Gene description for CD82
Gene name	CD82 molecule
Gene symbol	CD82
Other names/aliases	4F9 C33 GR15 IA4 KAI1 R2 SAR2 ST6 TSPAN27
Species	Homo sapiens
Experiment description of studies that identified CD82 in B cells
1 Experiment ID 16 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting Immunoelectron Microscopy PubMed ID 9685355    Organism Homo sapiens Experiment description Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. Authors Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ Journal name JBC Publication year 1998 Sample B cells Sample name RN (HLA-DR15+) Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.12-1.18 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Immunoelectron microscopy EV-TRACK -
2 Experiment ID 79 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 20458337    Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1 Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting EV-TRACK EV100035: EV-METRIC:44%
3 Experiment ID 80 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 20458337    Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2 Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting EV-TRACK EV100035: EV-METRIC:44%
4 Experiment ID 81 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 20458337    Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3 Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting EV-TRACK EV100035: EV-METRIC:44%
5 Experiment ID 271 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Immunoelectron microscopy PubMed ID 10903724    Organism Homo sapiens Experiment description Follicular dendritic cells carry MHC class II-expressing microvesicles at their surface Authors Denzer K, van Eijk M, Kleijmeer MJ, Jakobson E, de Groot C, Geuze HJ Journal name J Immunol Publication year 2000 Sample B cells Sample name RN Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Immunoelectron microscopy EV-TRACK -
6 Experiment ID 395 Identified molecule protein Extracellular vesicle type Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 19413345    Organism Homo sapiens Experiment description Proteomic analysis of malignant B-cell derived microparticles reveals CD148 as a potentially useful antigenic biomarker for mantle cell lymphoma diagnosis. Authors Miguet L, Béchade G, Fornecker L, Zink E, Felden C, Gervais C, Herbrecht R, Van Dorsselaer A, Mauvieux L, Sanglier-Cianferani S. Journal name J Proteome Res Publication year 2009 Sample B cells Sample name Patients of chronic lymphocytic leukemia-B cells Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
7 Experiment ID 396 Identified molecule protein Extracellular vesicle type Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 19413345    Organism Homo sapiens Experiment description Proteomic analysis of malignant B-cell derived microparticles reveals CD148 as a potentially useful antigenic biomarker for mantle cell lymphoma diagnosis. Authors Miguet L, Béchade G, Fornecker L, Zink E, Felden C, Gervais C, Herbrecht R, Van Dorsselaer A, Mauvieux L, Sanglier-Cianferani S. Journal name J Proteome Res Publication year 2009 Sample B cells Sample name Patients of mantle cell lymphoma-B cells Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
8 Experiment ID 397 Identified molecule protein Extracellular vesicle type Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 19413345    Organism Homo sapiens Experiment description Proteomic analysis of malignant B-cell derived microparticles reveals CD148 as a potentially useful antigenic biomarker for mantle cell lymphoma diagnosis. Authors Miguet L, Béchade G, Fornecker L, Zink E, Felden C, Gervais C, Herbrecht R, Van Dorsselaer A, Mauvieux L, Sanglier-Cianferani S. Journal name J Proteome Res Publication year 2009 Sample B cells Sample name Patients of small cell lymphoma-B cells Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
9 Experiment ID 534 Identified molecule protein Extracellular vesicle type Exosomes/Microvesicles/Oncosomes/Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 23818640    Organism Homo sapiens Experiment description Modulation of B-cell exosome proteins by gamma herpesvirus infection. Authors Meckes DG Jr, Gunawardena HP, Dekroon RM, Heaton PR, Edwards RH, Ozgur S, Griffith JD, Damania B, Raab-Traub N. Journal name Proc Natl Acad Sci U S A Publication year 2013 Sample B cells Sample name EBV- and KSHV-infected B cells (JSC-1, BC1) Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130064: EV-METRIC:25%
10 Experiment ID 535 Identified molecule protein Extracellular vesicle type Exosomes/Microvesicles/Oncosomes/Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 23818640    Organism Homo sapiens Experiment description Modulation of B-cell exosome proteins by gamma herpesvirus infection. Authors Meckes DG Jr, Gunawardena HP, Dekroon RM, Heaton PR, Edwards RH, Ozgur S, Griffith JD, Damania B, Raab-Traub N. Journal name Proc Natl Acad Sci U S A Publication year 2013 Sample B cells Sample name EBV-infected B cells (#1, HLJ, IM9, CP) Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130064: EV-METRIC:25%
11 Experiment ID 536 Identified molecule protein Extracellular vesicle type Exosomes/Microvesicles/Oncosomes/Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 23818640    Organism Homo sapiens Experiment description Modulation of B-cell exosome proteins by gamma herpesvirus infection. Authors Meckes DG Jr, Gunawardena HP, Dekroon RM, Heaton PR, Edwards RH, Ozgur S, Griffith JD, Damania B, Raab-Traub N. Journal name Proc Natl Acad Sci U S A Publication year 2013 Sample B cells Sample name KSHV-infected B cell (JC, BC3, BCP1, BCBL1) Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130064: EV-METRIC:25%
12 Experiment ID 537 Identified molecule protein Extracellular vesicle type Exosomes/Microvesicles/Oncosomes/Microparticles MISEV EVs Identification method Mass spectrometry PubMed ID 23818640    Organism Homo sapiens Experiment description Modulation of B-cell exosome proteins by gamma herpesvirus infection. Authors Meckes DG Jr, Gunawardena HP, Dekroon RM, Heaton PR, Edwards RH, Ozgur S, Griffith JD, Damania B, Raab-Traub N. Journal name Proc Natl Acad Sci U S A Publication year 2013 Sample B cells Sample name Normal B cells (BJAB) Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130064: EV-METRIC:25%
13 Experiment ID 545 Identified molecule protein Extracellular vesicle type Exosomes/Extracellular vesicles/Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23983189    Organism Homo sapiens Experiment description Characterization of Multiple Myeloma Vesicles by Label-Free Relative Quantitation. Authors Harshman SW, Canella A, Ciarlariello PD, Rocci A, Agarwal K, Smith EM, Talabere T, Efebera YA, Hofmeister CC, Benson DM Jr, Paulaitis ME, Freitas MA, Pichiorri F. Journal name Proteomics Publication year 2013 Sample B cells Sample name MM.1S Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130092: EV-METRIC:22%
14 Experiment ID 546 Identified molecule protein Extracellular vesicle type Exosomes/Extracellular vesicles/Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 23983189    Organism Homo sapiens Experiment description Characterization of Multiple Myeloma Vesicles by Label-Free Relative Quantitation. Authors Harshman SW, Canella A, Ciarlariello PD, Rocci A, Agarwal K, Smith EM, Talabere T, Efebera YA, Hofmeister CC, Benson DM Jr, Paulaitis ME, Freitas MA, Pichiorri F. Journal name Proteomics Publication year 2013 Sample B cells Sample name U266 Isolation/purification methods - Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK EV130092: EV-METRIC:22%